But sometimes this setup seems to work better than unstacked grid, esp when there is a central spanner.
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Lotame is unveiling what it says is a new approach to the data management business, with what it calls an "unstacked" strategy.
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We shuffle around on the buffed linoleum floor of the family visitation room where a few plastic chairs are unstacked for us.
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Unstacked, the beds become tables against which the men fling their own and others' bodies, as images of sacrifice and torture accumulate.
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Lotame pitches an 'unstacked' approach to selling data tools And far from being an attempt to circumvent privacy restrictions, Solomon argued that Cartographer actually makes it easier for publishers to stay compliant with Europe's GDPR and California's CCPA rules, because they can do a better job of storing a customer's privacy preferences.
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Units may move over friendly units while moving (never enemy units) but must end their movement unstacked.
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Coaxial stacking is the tendency of nucleic acid blunt ends to bind to each other, by interactions between the exposed bases. There are three possible conformers: an unstacked form and two stacked forms. The unstacked form dominates in the absence of divalent cations such as Mg2+, because of electrostatic repulsion between the negatively charged backbones of the strands. In the presence of at least about 0.1 mM Mg2+, the electrostatic repulsion is counteracted and the stacked structures predominate.
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Thylakoid structures Scanning transmission electron microscope (STEM) imaging of thylakoid membranes 10-nm-thick STEM tomographic slice from a lettuce chloroplast. Grana stacks are interconnected by unstacked stromal thylakoids, called “stroma lamellae”. Scalebar = 200 nm. See .
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A gerontoplast is a plastid that develops from a chloroplast during the senescing of plant foliage. Gerontoplast development is generally seen to be the process of grana being unstacked, loss of thylakoid membranes, and large accumulation of plastoglobuli.
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In contrast, a double-loop structure with an unusual unstacked DNA conformation at its downstream edge was observed when the extruded bases were positioned at the primer–template junction, showing that misalignments can be modified by neighboring DNA secondary structure.
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Four cubes with open tops and bottoms are stacked to form a cabinet. An assistant stands inside and the cabinet is closed. Metal blades are slid into the cabinet, apparently slicing the assistant into four pieces, and closing the top and bottom of each cube. The cubes are then unstacked, and restacked in a different order.
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Heat denaturation of DNA, also called melting, causes the double helix structure to unwind to form single stranded DNA. When DNA in solution is heated above its melting temperature (usually more than 80 °C), the double- stranded DNA unwinds to form single-stranded DNA. The bases become unstacked and can thus absorb more light. In their native state, the bases of DNA absorb light in the 260-nm wavelength region.
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When the bases become unstacked, the wavelength of maximum absorbance does not change, but the amount absorbed increases by 37%. A double stranded DNA strand dissociating to two single strands produces a sharp cooperative transition. Hyperchromicity can be used to track the condition of DNA as temperature changes. The transition/melting temperature (Tm) is the temperature where the absorbance of UV light is 50% between the maximum and minimum, i.e.
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Glaucophyte chloroplasts also contain concentric unstacked thylakoids, which surround a carboxysome – an icosahedral structure that glaucophyte chloroplasts and cyanobacteria keep their carbon fixation enzyme RuBisCO in. The starch that they synthesize collects outside the chloroplast. Like cyanobacteria, glaucophyte and rhodophyte chloroplast thylakoids are studded with light collecting structures called phycobilisomes. For these reasons, glaucophyte chloroplasts are considered a primitive intermediate between cyanobacteria and the more evolved chloroplasts in red algae and plants.
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All products, regardless of cleavage, are heteroduplexes in the region of Holliday junction migration. Many proteins are able to recognize or distort the Holliday junction structure. One such class contains junction-resolving enzymes that cleave the junctions, sometimes in a sequence-specific fashion. Such proteins distort the structure of the junction in various ways, often pulling the junction into an unstacked conformation, breaking the central base pairs, and/or changing the angles between the four arms.
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Instead of having localized stacks of thylakoids, Trichodesmium has unstacked thylakoids found throughout the cell. Trichodesmium is highly vacuolated and the content and size of the vacuoles shows diurnal variation. Large gas vesicles (either along the periphery as seen in T. erythaeum or found distributed throughout the cell as seen in T. thiebautii) allow Trichodesmium to regulate buoyancy in the water column. These gas vesicles can withstand high pressure, presumably those up to 100 – 200 m in the water column, allowing Trichodesmium to move vertically through the water column harvesting nutrients.
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Unlike the tandem G-U motif, the A-A platform motif binds preferentially to monovalent cations. In many of these motifs, absence of the monovalent or divalent cations results in either greater flexibility or loss of tertiary structure. Divalent metal ions, especially magnesium, have been found to be important for the structure of DNA junctions such as the Holliday junction intermediate in genetic recombination. The magnesium ion shields the negatively charged phosphate groups in the junction and allows them to be positioned closer together, allowing a stacked conformation rather than an unstacked conformation.
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As of 2000, it was not known with certainty whether the electrostatic shielding was the result of site-specific binding of cations to the junction, or the presence of a diffuse collection of the ions in solution. The unstacked form is a nearly square planar, extended conformation. On the other hand, the stacked conformers have two continuous double-helical domains separated by an angle of about 60° in a right-handed direction. Two of the four strands stay roughly helical, remaining within each of the two double-helical domains, while the other two cross between the two domains in an antiparallel fashion.
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In junctions with symmetrical sequences, the branchpoint is mobile and can migrate in a random walk process. The rate of branch migration varies dramatically with ion concentration, with single- step times increasing from 0.3−0.4 ms with no ions to 270−300 ms with 10 mM Mg2+. The change in rate is correlated with the formation of the stacked versus the unstacked structures. Holliday junctions with a nick, or break in one of the strands, at the junction point adopt a perpendicular orientation, and always prefer the stacking conformer that places the nick on a crossover strand rather than a helical strand.
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This bead is slid down the two suspension cords to the top of the inro to hold the stack together while the inro is worn, and slid up to the netsuke when the boxes need to be unstacked to access their contents. Inro are mostly made from paper, wood, metal, or ivory, with the most common material being paper. Paper inro are made by winding and hardening many layers of washi paper with lacquer; paper was a popular material for inro as unlike wood, it would not distort and crack over time. Inro are commonly decorated with lacquered designs, with the most expensively-produced inro featuring , , ivory inlay and metal foiling.
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Approximately 5% of the red algae occur in freshwater environments with greater concentrations found in warmer areas. Except for two coastal cave dwelling species in the asexual class Cyanidiophyceae, there are no terrestrial species, which may be due to an evolutionary bottleneck where the last common ancestor lost about 25% of its core genes and much of its evolutionary plasticity.Why don't we live on a red planet? The red algae form a distinct group characterized by having eukaryotic cells without flagella and centrioles, chloroplasts that lack external endoplasmic reticulum and contain unstacked (stroma) thylakoids, and use phycobiliproteins as accessory pigments, which give them their red color.
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Beach noted that early testing with the unstacked cities on archipelagos generated by their older system made gameplay nearly impossible, and that with mountains becoming a valuable resource towards city expansion, test players would restart maps built on the old map generation system to get the right placement of mountains to exploit them successfully. The new map generation system attempts to spread out terrain more, and in areas where one type of important terrain may be absent, makes up for this by including other valuable terrain spaces, such as a river-rich region where there is a lack of mountains. Senior gameplay designer Anton Strenger compared their approach towards the development of the computer opponents, with main and hidden agendas, similar to concepts they had used in Rising Tide expansion for Civilization: Beyond Earth. They selected historical leaders to span a diverse range of faction and playstyle dynamics, while also looking for figures that had "really interesting personalities" that they could fit these agendas into.
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