2 Sentences With "phosphorescing"

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In some "writing" schemes, the tagged molecule ends up in an excited state. If the molecule relaxes through phosphorescence, lasting long enough to see line displacement, this can be used to track the written line and no additional visualisation step is needed. If during tagging the molecule did not reach a phosphorescing state, or relaxed before the molecule was "read", a second step is needed. The tagged molecule is then excited using a second laser beam, employing a wavelength such that it specifically excites the tagged molecule.
Fluorescence microscopy relies upon fluorescent compounds, or fluorophores, in order to image biological systems. Since fluorescence and phosphorescence are competitive methods of relaxation, a fluorophore that undergoes intersystem crossing to the triplet excited state no longer fluoresces and instead remains in the triplet excited state, which has a relatively long lifetime, before phosphorescing and relaxing back to the singlet ground state so that it may continue to undergo repeated excitation and fluorescence. This process in which fluorophores temporarily do not fluoresce is called blinking. While in the triplet excited state, the fluorophore may undergo photobleaching, a process in which the fluorophore reacts with another species in the system, which can lead to the loss of the fluorescent characteristic of the fluorophore.