How to use metho in a sentence? Find typical usage patterns (collocations)/phrases/context for "metho" and check conjugation/comparative form for "metho". Mastering all the usages of "metho" from sentence examples published by news publications.
"RIP Metho Tom". Ozbike: Thunder Down Under. no. 320, 18–22.Veno, Arthur (2004).
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"RIP Metho Tom". Ozbike: Thunder Down Under. no. 320, 18–22.Veno, Arthur (2004).
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Reference dose for methanol is 0.5 mg/kg/day.Methanol (CASRN 67-56-1) Ethanol is sometimes denatured (adulterated), and made poisonous, by the addition of methanol. The result is known as methylated spirit, "meths" (British use) or "metho" (Australian slang). This is not to be confused with "meth", a common abbreviation for methamphetamine and for methadone in Britain and the United States.
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" Ian McFarlane said, "Teenage Love proved to be a remarkable collection of songs that were brimming with loose ends and vibrant spontaneity. Outstanding tracks like "Metho Blues", "Nothing but You", "Teenage Love Affair" and the boogie "Drinkin' in Port Lincoln" possessed more grit and fire than most of the debut album or Twentieth Century. The album reached the number 6 in the Australian charts.
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There are three major techniques for the site-specific mapping of pseudouridine in RNA, called Pseudo-seq, Ψ-seq and PSI-seq. All these methods are based on the unique reaction between pseudouridine and N-cyclohexyl-N'-(2-morpholinoethyl)carbodiimide metho-p- toluenesulfonate (CMCT). The RNA to be analyzed is fragmented and incubated with CMCT. Even if CMCT can form covalent bonds with U, G and Ψ residues, only Ψ-CMC is resistant to alkaline hydrolysis (U-CMC and G-CMC get hydrolyzed).
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There are multiple pseudouridine detection methods beginning with the addition of N-cyclohexyl-N′-b-(4-methylmorpholinium) ethylcarbodiimide metho-p-toluene-sulfonate (CMCT; also known as CMC), since its reaction with pseudouridine produces CMC-Ψ. CMC-Ψ causes reverse transcriptase to stall one nucleotide in the 3’ direction. These methods have single-nucleotide resolution. In an optimisation step, azido-CMC can confer the ability to add biotinylation; subsequent biotin pulldown will enrich Ψ-containing transcripts, allowing identification of even low-abundance transcripts.
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Structure of CMCT used in RNA structure probing Mechanism of the reaction between uracil and carbodiimides The carbodiimide moiety can also form covalent adducts at exposed nucleobases, which are uracil, and to a smaller extent guanine, upon nucleophilic attack by a deprotonated N. They react primarily with N3 of uracil and N1 of guanine modifying two sites responsible for hydrogen bonding on the bases. 1-cyclohexyl-(2-morpholinoethyl)carbodiimide metho-p-toluene sulfonate, also known as CMCT or CMC, is the most commonly used carbodiimide for RNA structure probing. Similar to DMS, it can be detected by reverse transcription followed by gel electrophoresis or high-throughput sequencing. As it is reactive towards G and U, it can be used to complement the data from DMS probing experiments, which inform A and C. 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, also known as EDC, is a water- soluble carbodiimide that exhibits similar reactivity as CMC, and is also used for the chemical probing of RNA structure.
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