How to use explant culture in a sentence? Find typical usage patterns (collocations)/phrases/context for "explant culture" and check conjugation/comparative form for "explant culture". Mastering all the usages of "explant culture" from sentence examples published by news publications.
Vitamins are also provided to enhance growth such as Gamborg B5 vitamins. For plant cells, enrichment with nitrogen, phosphorus, and potassium is especially important. Plant callus is usually derived from somatic tissues. The tissues used to initiate callus formation depends on plant species and which tissues are available for explant culture.
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Besides normal cultivation, D. thapsi has been propagated using explant culture, a technique employed for the isolation of cells from piece(s) of tissue for harvesting. Auxins NAA, 2,4-D and IAA alone or combined with BA produced a callus. NAA caused root formation and BA shoot formation. NAA and BA combined induced organ generation much effectively.
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15d-Δ12,14-PGJ2 and its PGD2 precursor have been demonstrated to suppress hair growth in studies of mouse and human follicular explant culture models; further studies examining the content of these two prostaglanidns in normal and balding tissue of mice and humans have implicated PGD2 and to a much lesser extent 15d-Δ12,Δ14-PGJ2 in the development of male pattern baldness.
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This method is known as explant culture. Cells that are cultured directly from a subject are known as primary cells. With the exception of some derived from tumors, most primary cell cultures have limited lifespan. An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene.
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Expression of these genes answered the age-old question of when the embryo decides to make a nervous system: Sive showed that future brain cells are set aside when the embryo is just a ball of cells. Function of these genes, including otx2 and zic1 (opl), was studied using hormone-inducible fusion proteins, a technique first used in embryos by Sive. She also developed the first zebrafish ‘explant’ culture method, and so identified cell interactions that initiate brain development. As well, Sive identified retinoic acid as a regulator of brain patterning, and demonstrated its activity on expression of hindbrain Hox genes.
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